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Biochemistry

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JoVE Journal Biochemistry
Mass Spectrometry and Luminogenic-based Approaches to Characterize Phase I Metabolic Competency of In Vitro Cell Cultures
 

Mass Spectrometry and Luminogenic-based Approaches to Characterize Phase I Metabolic Competency of In Vitro Cell Cultures

Article DOI: 10.3791/55502-v • 10:44 min • March 28th, 2017
March 28th, 2017 •
1, 1
1Research and Development, British American Tobacco

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Metabolic competency of in vitro systems is a key requirement for the biotransformation and disposition of drugs and toxicants. In this protocol, we describe the application of reference metabolic probes to assess phase I metabolism in cell cultures.

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Keywords: Mass Spectrometry Luminogenic Phase I Metabolism In Vitro Cell Cultures Cytochrome P450 CYP Enzymes Probe Substrates Inhibitors Hepatic Cell Line Williams' E Medium Air-liquid Interface Transepithelial Resistance Cilia Beat Frequency
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