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Immunology and Infection

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JoVE Journal Immunology and Infection
Immunofluorescence Analysis of Stress Granule Formation After Bacterial Challenge of Mammalian Cells
 

Immunofluorescence Analysis of Stress Granule Formation After Bacterial Challenge of Mammalian Cells

Article DOI: 10.3791/55536-v • 11:37 min • July 3rd, 2017
July 3rd, 2017 •
1, 1,2, 3
1Unité de Pathogénie Microbienne Moléculaire, INSERM U786, Institut Pasteur, 2Microbiologie et Maladies Infectieuses, Collège de France, 3Laboratory of Intestinal Immunity, Institut Imagine-INSERM UMR 1163, Université Paris Descartes-Sorbonne Paris Cité

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Resumo
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We describe a method for the qualitative and quantitative analysis of stress granule formation in mammalian cells after the cells are challenged with bacteria and a number of different stresses. This protocol can be applied to investigate the cellular stress granule response in a wide range of host-bacterial interactions.

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Keywords: Immunofluorescence Stress Granule Bacterial Challenge Mammalian Cells Cellular Microbiology Pathogen Stress Granule Pathway Image Analysis S.flexneri M90T Tryptic Soy Agar Tryptic Soy Broth Virulence Gene Expression HeLa Cells Infection Medium Gentamicin
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