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Immunology and Infection

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JoVE Journal Immunology and Infection
Small RNA Transfection in Primary Human Th17 Cells by Next Generation Electroporation
 

Small RNA Transfection in Primary Human Th17 Cells by Next Generation Electroporation

Article DOI: 10.3791/55546-v • 10:15 min • April 13th, 2017
April 13th, 2017 •
1, 1
1Department of Microbiology & Immunology, Sandler Asthma Basic Research Center, University of California, San Francisco

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Resumo
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Next generation electroporation is an efficient method for transfecting human Th17 cells with small RNAs to alter gene expression and cell behavior.

Tags

Small RNA Transfection Primary Human Th17 Cells Next Generation Electroporation T Cell Biology Gene Regulation Immune Function Synthetic Small RNA Reagents Lymphocytes Antihuman CD3 Antihuman CD28 Cord-blood Mononuclear Cells CBMCs CD4 Positive T Cell Isolation Isolation Buffer
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