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Analyse for å måle nukleocytoplasmisk transport i sanntid innen Motor Neuron-lignende NSC-34-celler
 
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Analyse for å måle nukleocytoplasmisk transport i sanntid innen Motor Neuron-lignende NSC-34-celler

Article DOI: 10.3791/55676-v • 08:53 min • May 16th, 2017
May 16th, 2017 •
1, 1, 1,2
1Department of Physiology and Cell Biology, Faculty of Health Sciences, Ben-Gurion University of the Negev, 2The Zlotowski Center for Neuroscience, Ben-Gurion University of the Negev

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Denne protokollen beskriver en metode for sensitiv måling av nukleocytoplasmatisk transportrate i motorneuronlignende NSC-34-celler ved å kvantifisere sanntidsendringen i nukleær import av et NLS-NES-GFP-protein.

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Neurovitenskap utgave 123 nukleocytoplasmisk transport shuttle GFP NSC-34-celler C9orf72 ALS
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