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JoVE Journal Bioengineering
An Integrated System to Remotely Trigger Intracellular Signal Transduction by Upconversion Nanoparticle-mediated Kinase Photoactivation
 

An Integrated System to Remotely Trigger Intracellular Signal Transduction by Upconversion Nanoparticle-mediated Kinase Photoactivation

Article DOI: 10.3791/55769-v • 11:20 min • August 30th, 2017
August 30th, 2017 •
1,2, 1, 1,2, 3, 1,2, 1
1Institute of Chemistry, Academia Sinica, 2Department of Chemistry, National Taiwan University, 3Department of Materials and Mineral Resources Engineering, National Taipei University of Technology

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Resumo
Automatic Translation

In this protocol, caged protein kinase A (PKA), a cellular signal transduction bioeffector, was immobilized on a nanoparticle surface, microinjected into the cytosol, and activated by the upconverted UV light from near-infrared (NIR) irradiation, inducing downstream stress fiber disintegration in the cytosol.

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Keywords: Upconversion Nanoparticles Photoactivation Kinase Near-infrared Signal Transduction Fluorescence Spectrometer Optical Setup
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