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JoVE Journal Developmental Biology
Expansion and Adipogenesis Induction of Adipocyte Progenitors from Perivascular Adipose Tissue Isolated by Magnetic Activated Cell Sorting
 

Expansion and Adipogenesis Induction of Adipocyte Progenitors from Perivascular Adipose Tissue Isolated by Magnetic Activated Cell Sorting

Article DOI: 10.3791/55818-v • 08:28 min • June 30th, 2017
June 30th, 2017 •
1, 2, 2, 1
1Department of Large Animal Clinical Sciences, Michigan State University, 2Department of Pharmacology and Toxicology, Michigan State University

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Here we report a method for isolation of Adipocyte Progenitor Cell (APC) populations from Perivascular Adipose Tissue (PVAT) using Magnetic-activated Cell Sorting (MCS). This method allows for an increased isolation of APC per gram of adipose tissue when compared to Fluorescence-Activated Cell Sorting (FACS).

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Keywords: Adipocyte Progenitor Cells Perivascular Adipose Tissue Magnetic Activated Cell Sorting Adipogenesis Adipose Tissue Isolation Stromal Vascular Fraction Collagenase Digestion Cell Filtration Erythrocyte Lysis
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