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大肠癌细胞中磷酸化和微管结合与 Tau 蛋白定位的测定
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Bioquímica
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JoVE Journal Bioquímica
Assay for Phosphorylation and Microtubule Binding Along with Localization of Tau Protein in Colorectal Cancer Cells
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大肠癌细胞中磷酸化和微管结合与 Tau 蛋白定位的测定

DOI:
10.3791/55932-v

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12:55 min

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October 10, 2017

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, ,
1Systems Biotechnology Research Center,KIST Gangneung Institute of Natural Products, 2Division of Bio-Medical Science & Technology, KIST School,Korea University of Science and Technology

Capítulos

  • 00:05Título
  • 01:01Cell Culture, Treatment, and Examination of Protein Expression
  • 02:53Phosphate Assay
  • 05:22Microtubule-binding Assay
  • 07:55Localization and Expression of Tau After Treatment of Cells with Curcumin
  • 10:23Results: Phosphorylation, Microtubule Binding, and Localization of Tau Protein in Colorectal Cancer Cells
  • 12:03Conclusion

Summary

Tadução automática

Tadução automática

本手稿描述了测量 tau tau, 测量 tau 结合到微管的标准协议, 以及药物治疗后细胞内 tau 的定位。这些协议可以重复使用, 以筛选药物或其他化合物的目标 tau 或微管结合。

Tags

Tau PhosphorylationMicrotubule BindingTau LocalizationConfocal MicroscopyColorectal Cancer CellsPhosphatase AssayMicrotubule-binding AssayTau ProteinHCT 116 CellsCurcuminLithium ChlorideRIPA BufferBradford AssayAlkaline Phosphatase

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