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リン酸化と大腸癌細胞におけるタウ蛋白の局在と微小管結合の試金
JoVE Journal
Bioquímica
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JoVE Journal Bioquímica
Assay for Phosphorylation and Microtubule Binding Along with Localization of Tau Protein in Colorectal Cancer Cells
DOI:

12:55 min

October 10, 2017

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Capítulos

  • 00:05Título
  • 01:01Cell Culture, Treatment, and Examination of Protein Expression
  • 02:53Phosphate Assay
  • 05:22Microtubule-binding Assay
  • 07:55Localization and Expression of Tau After Treatment of Cells with Curcumin
  • 10:23Results: Phosphorylation, Microtubule Binding, and Localization of Tau Protein in Colorectal Cancer Cells
  • 12:03Conclusion

Summary

Tadução automática

本稿では、測定、微小管と薬物治療に続く細胞内のタウのローカリゼーションのタウ結合タウ タウを測定するための標準プロトコルについて説明します。これらのプロトコルは、薬やタウ タウや微小管結合を対象とする他の化合物をスクリーニングするため繰り返し使用できます。

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