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Recording Synaptic Plasticity in Acute Hippocampal Slices Maintained in a Small-volume Recycling-, Perfusion-, and Submersion-type Chamber System
 

Recording Synaptic Plasticity in Acute Hippocampal Slices Maintained in a Small-volume Recycling-, Perfusion-, and Submersion-type Chamber System

Article DOI: 10.3791/55936-v • 09:52 min • January 1st, 2018
January 1st, 2018 •
1, 1, 1, 1
1The Institutes of Brain Science, the State Key Laboratory of Medical Neurobiology, the Collaborative Innovation Center for Brain Science, Fudan University

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This protocol describes the stabilization of the oxygen level in a small volume of recycled buffer and methodological aspects of recording activity-dependent synaptic plasticity in submerged acute hippocampal slices.

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Keywords: Synaptic Plasticity Acute Hippocampal Slices Recycling-perfusion-submersion Chamber Carbogenation Field Potential Recordings Neuroplasticity Synaptic Transmission Memory Formation Oxygen Stabilization Acute Slice Preparation Vibratome Hippocampal Formation Recycling System Carbogen Supply
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