Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Neuroscience / Determining Cell-surface Expression and Endocytic …
JoVE Journal
Neuroscience

É necessário ter uma assinatura JoVE para assistir este Faça login ou comece sua avaliação gratuita.

JoVE Journal Neuroscience
Determining Cell-surface Expression and Endocytic Rate of Proteins in Primary Astrocyte Cultures Using Biotinylation
 

Determining Cell-surface Expression and Endocytic Rate of Proteins in Primary Astrocyte Cultures Using Biotinylation

Article DOI: 10.3791/55974-v • 10:42 min • July 3rd, 2017
July 3rd, 2017 •
1, 1
1Department of Cellular and Physiological Sciences, University of British Columbia

Capítulos

Resumo
Automatic Translation

Two biotinylation-based methods, designed for determining the cell-surface expression and endocytic rate of proteins expressed at the plasma membrane, are presented in this report.

Tags

Cell-surface Expression Endocytic Rate Proteins Primary Astrocyte Cultures Biotinylation Cell-surface Biotinylation Pulse-chase Biotinylation Astrocytes Aquaporin-4 Cell Types Suspension Cells Adherent Cells Transmembrane Protein Extracellular Domain Laminin Water Permeable Channel Plasma Membrane Incubator Medium Aspiration CM-PBS Biotin Buffer Quenching Buffer
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter