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JoVE Journal Developmental Biology
In Vitro Ovule Cultivation for Live-cell Imaging of Zygote Polarization and Embryo Patterning in Arabidopsis thaliana
 

In Vitro Ovule Cultivation for Live-cell Imaging of Zygote Polarization and Embryo Patterning in Arabidopsis thaliana

Article DOI: 10.3791/55975-v • 05:41 min • September 11th, 2017
September 11th, 2017 •
*1,2, *1, 1,2,3, 1,3
1Division of Biological Science, Graduate School of Science, Nagoya University, 2Higashiyama Live-Holonics Project, JST-ERATO, Nagoya University, 3Institute of Transformative Bio-Molecules (ITbM), Nagoya University
* These authors contributed equally

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This manuscript describes an in vitro ovule cultivation method that enables live-cell imaging of Arabidopsis zygotes and embryos. This method is utilized to visualize the intracellular dynamics during zygote polarization and the cell fate specification in developing embryos.

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Keywords: In Vitro Ovule Cultivation Live-cell Imaging Zygote Polarization Embryo Patterning Arabidopsis Thaliana PDMS Micropillar Array N5T Medium Silique Dissection Ovule Transfer Cover Glass
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