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JoVE Journal Developmental Biology
Characterization of Thymic Settling Progenitors in the Mouse Embryo Using In Vivo and In Vitro Assays
 

Characterization of Thymic Settling Progenitors in the Mouse Embryo Using In Vivo and In Vitro Assays

Article DOI: 10.3791/52795-v • 08:56 min • June 9th, 2015
June 9th, 2015 •
1,3, 2, 3, 3, 3, 3
1Research Center Growth and Signaling, INSERM U845, Institut Cochin, 2Unit for Biology of Lymphocyte Populations, Immunology Department, Institut Pasteur and CIMI, Unity of Treg Biology and Therapy, University of Pierre & Marie Curie, 3Unit for Lymphopoiesis, Immunology Department, INSERM U668, University Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, Institut Pasteur

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This article describes in vivo and in vitro methodology to characterize the thymic settling progenitors by the analysis of the kinetics of generation, phenotype and numbers of their T cell progeny.

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Keywords: Thymic Settling Progenitors T Cell Development Lymphopenic Patients Fetal Thymic Organ Culture CD45 Allotypic Markers Dendritic Epithelial T Cells Peripheral Immune System Thymus Grafting CD3-/- Mice
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