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Cryopreservation of Zebrafish Spermatogonia by Whole Testes Needle Immersed Ultra-Rapid Cooling
 

Cryopreservation of Zebrafish Spermatogonia by Whole Testes Needle Immersed Ultra-Rapid Cooling

Article DOI: 10.3791/56118-v • 13:33 min • March 4th, 2018
March 4th, 2018 •
1, 1, 1, 1, 1, 1
1Department of Aquaculture, Szent István University

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The main purpose of this study was to adapt the needle immersed vitrification (NIV) procedure to cryopreserve whole zebrafish testes. Additionally, the repeatability of the method in five different zebrafish strains was tested.

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Keywords: Cryopreservation Zebrafish Spermatogonia Vitrification Whole Testes Ultra-rapid Cooling Germ Cells Transplantation Slow-rate Freezing Spermatozoa Euthanasia Dissection Testes Removal Sterilization Equilibration Vitrification Solutions
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