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Immunology and Infection

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JoVE Journal Immunology and Infection
Utilization of Capsules for Negative Staining of Viral Samples within Biocontainment
 

Utilization of Capsules for Negative Staining of Viral Samples within Biocontainment

Article DOI: 10.3791/56122-v • 08:23 min • July 19th, 2017
July 19th, 2017 •
1, 1, 1, 1, 2, 2, 2, 3, 1
1Pathology Division, United States Army Medical Research Institute of Infectious Diseases (USAMRIID), 2Diagnostic Systems Division, United States Army Medical Research Institute of Infectious Diseases (USAMRIID), 3Microscopy Innovations LLC

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This protocol provides instruction for negative staining virus samples which can easily be used in BSL-2, -3, or -4 laboratories. It includes the use of an innovative processing capsule, which protects the transmission electron microscopy grid and provides the user easier handling in the more turbulent environments within biocontainment.

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Capsules Negative Staining Viral Samples Biocontainment TEM Electron Microscopy Virology Morphology Structures Handling Grids TEM Diagnosis Unknown Virus Nanoparticles EM Investigation
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