Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Developmental Biology / Integration Free Derivation of Human Induced…
JoVE Journal
Developmental Biology

É necessário ter uma assinatura JoVE para assistir este Faça login ou comece sua avaliação gratuita.

JoVE Journal Developmental Biology
Integration Free Derivation of Human Induced Pluripotent Stem Cells Using Laminin 521 Matrix
 

Integration Free Derivation of Human Induced Pluripotent Stem Cells Using Laminin 521 Matrix

Article DOI: 10.3791/56146-v • 10:48 min • July 7th, 2017
July 7th, 2017 •
1, 1,2, 1, 1, 1, 1
1Department of Neuroscience, Karolinska Institutet, 2Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet

Capítulos

Resumo
Automatic Translation

Robust derivation of human induced pluripotent stem (hiPS) cells was achieved by using non-integrating Sendai virus (SeV) vector mediated reprogramming of dermal fibroblasts. hiPS cell maintenance and clonal expansion was performed using xeno-free and chemically defined culture conditions with recombinant human laminin 521 (LN-521) matrix and Essential E8 (E8) Medium.

Tags

Keywords: Human Induced Pluripotent Stem Cells Integration-free Derivation Laminin 521 Matrix Skin Biopsy Dispase Collagenase I Xeno-free Chemically Defined Culture System Disease Modeling Drug Screening Cell Therapy
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter