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Biochemistry
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Simple Elimination of Background Fluorescence in Formalin-Fixed Human Brain Tissue for Immunofluorescence Microscopy
 

Simple Elimination of Background Fluorescence in Formalin-Fixed Human Brain Tissue for Immunofluorescence Microscopy

Article DOI: 10.3791/56188-v 16:31 min September 3rd, 2017
September 3rd, 2017

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Resumo

Background autofluorescence of biological samples often complicates fluorescence-based imaging techniques, especially in aged human postmitotic tissues. This protocol describes how autofluorescence from these samples can be effectively removed using a commercially available light emitting diode light source to photobleach the sample prior to immunostaining.

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Keywords: Immunofluorescence Microscopy Autofluorescence Photobleaching LED Desk Lamp Formalin-fixed Human Brain Tissue FTLD-T Orbital Frontal Gyri Tissue Preparation Amino Staining Cold Room Azide TBS
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