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JoVE Journal Genetics
Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage
 

Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage

Article DOI: 10.3791/56213-v • 10:44 min • January 31st, 2018
January 31st, 2018 •
*1, *2, 2, 2, 2, 2,3,4, 1
1Department of Biological Chemistry, School of Medicine, University of California, Irvine, 2Beckman Laser Institute and Medical Clinic, University of California, Irvine, 3Department of Developmental and Cell Biology, School of Biological Sciences, University of California, Irvine, 4Department of Biomedical Engineering and Surgery, University of California, Irvine
* These authors contributed equally

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The goal of this protocol is to describe how to use laser microirradiation to induce different types of DNA damage, including relatively simple strand breaks and complex damage, to study DNA damage signaling and repair factor assembly at damage sites in vivo.

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Laser Microirradiation DNA Damage DNA Repair DNA Damage Signaling Repair Factor Assembly Strand Breaks Complex Damage Macromolecular Dynamics Cell Nucleus Cellular Responses Factor Recruitment Modifications Fluorescently-tagged Proteins Hela Cells Culture Medium
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