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JoVE Journal Genetics
Use of Frozen Tissue in the Comet Assay for the Evaluation of DNA Damage
 

Use of Frozen Tissue in the Comet Assay for the Evaluation of DNA Damage

Article DOI: 10.3791/59955-v • 11:30 min • March 24th, 2020
March 24th, 2020 •
*1, 1, 1, 2
1Toxicology Program, ILS, Inc., 2Division of the National Toxicology Program, National Institute of Environmental Health Sciences
* These authors contributed equally

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This protocol describes several procedures for preparing high quality frozen tissue samples at the time of necropsy for use in the comet assay to assess DNA damage: 1) minced tissue, 2) scraped epithelial cells from the gastrointestinal tract, and 3) cubed tissue samples, requiring homogenization using a tissue mincing device.

Tags

Frozen Tissue Comet Assay DNA Damage Evaluation Logistics Remote Laboratory Tissue Analysis Personnel Training Technical Errors Mechanical DNA Damage Pre-clinical Drug Development Safety Assessment Environmental Bio-monitoring Genotoxic Potential DNA Repair Protective Effects Chemotherapy Radiation Food Additives
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