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JoVE Journal Bioengineering
Dendrimer-based Uneven Nanopatterns to Locally Control Surface Adhesiveness
 

Dendrimer-based Uneven Nanopatterns to Locally Control Surface Adhesiveness: A Method to Direct Chondrogenic Differentiation

Article DOI: 10.3791/56347-v • 14:47 min • January 20th, 2018
January 20th, 2018 •
1,2, 3,1, 1, 4,5, 4,5, 4,5, 6, 3,6, 1,3,7, 8,3, 8,3,5, 1,3,2
1Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology (BIST), 2Department of Engineering Electronics, University of Barcelona (UB), 3Networking Biomedical Research Center (CIBER), 4Instituto de Investigacin Biomédica de Málaga (IBIMA), Department of Organic Chemistry, Universidad de Málaga (UMA), 5Andalusian Centre for Nanomedicine and Biotechnology-BIONAND, 6Unidad de Bioingeniería Tisular y Terapia Celular (GBTTC-CHUAC), Grupo de Reumatolog ía, Instituto de Investigación Biomèdica de A Coruña (INIBIC), Complexo Hospitalario Universitario de A Coruña (CHUAC), Sergas, Universidade da Coruña (UDC), 7Institució Catalana de Recerca i Estudis Avançats (ICREA), 8Instituto de Investigación Biomédica de Málaga (IBIMA), Department of Cell Biology, Genetics and Physiology, Universidad de Málaga (UMA)

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Resumo
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A method to obtain dendrimer-based uneven nanopatterns that permit the nanoscale control of local arginine-glycine-aspartic acid (RGD) surface density is described and applied for the study of cell adhesion and chondrogenic differentiation.

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Keywords: Dendrimer Nanopattern Surface Adhesiveness Chondrogenic Differentiation RGD Peptide PLLA Spin Coating UV Sterilization Cell Adhesion Microscopy Immunostaining
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