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Monitoring the Effect of Osmotic Stress on Secretory Vesicles and Exocytosis
 

Monitoring the Effect of Osmotic Stress on Secretory Vesicles and Exocytosis

Article DOI: 10.3791/56537-v • 08:08 min • February 19th, 2018
February 19th, 2018 •
1, 1, 2, 1
1Department of Chemistry and Chemical Engineering, Chalmers University of Technology, 2Department of Chemistry and Molecular Biology, University of Gothenburg

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Resumo
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Osmotic stress affects exocytosis and the amount of neurotransmitter released during this process. We demonstrate how combining electrochemical methods together with transmission electron microscopy can be used to study the effect of extracellular osmotic pressure on exocytosis activity, vesicle quantal size, and the amount of neurotransmitter released during exocytosis.

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Keywords: - Osmotic Stress - Secretory Vesicles - Exocytosis - Amperometric Recording - Carbon Fiber Microelectrode - Cyclic Voltammetry - Chromaffin Cells - Patch Clamp - Faraday Cage - Microscope Heating Stage
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