/
/
Détection visuelle de plusieurs acides nucléiques dans une gamme capillaire
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
1Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Center for Systems Biomedicine,Shanghai Jiao Tong University, 2State Key Laboratory of Oncogenes and Related Genes, 3School of Biomedical Engineering,Shanghai Jiao Tong University, 4Collaborative Innovation Center for Biosafety of GMOs, National Center for the Molecular Characterization of Genetically Modified Organisms, School of Life Sciences and Biotechnology,Shanghai Jiao Tong University, 5Key Laboratory of Crop Marker-Assisted Breeding of Huaian Municipality,Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental Protection, 6Department of Biomedical Engineering, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, School of Medicine,Tsinghua University
Capítulos
- 00:05Título
- 00:33Fabrication of the Capillary Array-based Ready-to-use Cassette
- 03:55Performance of LAMP Reaction in Capillary Array
- 05:07Results Readout and Data Analysis
- 07:01Results: The Specificity of the Cassette to Monitor GMOs
- 08:21Conclusion
Ce protocole décrit la fabrication d’une cassette de petite, prêt à l’emploi qui peut être appliquée pour la détection visuelle de plusieurs acides nucléiques lors d’un test unique, qui est facile à utiliser. Dans cette approche, une gamme capillaire a été utilisée pour multiplexe et très efficace de détection des cibles de l’OGM.
