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1Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Center for Systems Biomedicine,Shanghai Jiao Tong University, 2State Key Laboratory of Oncogenes and Related Genes, 3School of Biomedical Engineering,Shanghai Jiao Tong University, 4Collaborative Innovation Center for Biosafety of GMOs, National Center for the Molecular Characterization of Genetically Modified Organisms, School of Life Sciences and Biotechnology,Shanghai Jiao Tong University, 5Key Laboratory of Crop Marker-Assisted Breeding of Huaian Municipality,Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental Protection, 6Department of Biomedical Engineering, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, School of Medicine,Tsinghua University
Capítulos
- 00:05Título
- 00:33Fabrication of the Capillary Array-based Ready-to-use Cassette
- 03:55Performance of LAMP Reaction in Capillary Array
- 05:07Results Readout and Data Analysis
- 07:01Results: The Specificity of the Cassette to Monitor GMOs
- 08:21Conclusion
Questo protocollo descrive la realizzazione di una cassetta piccola, ready-to-use che possa essere applicata per rilevamento visivo degli acidi nucleici multipli in un singolo, test che è facile da usare. In questo approccio, una matrice capillare è stata utilizzata per il rilevamento di multiplex e altamente efficiente di bersagli di OGM.
