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A Quantitative Dot Blot Assay for AAV Titration and Its Use for Functional Assessment of the Adeno-associated Virus Assembly-activating Proteins
 

A Quantitative Dot Blot Assay for AAV Titration and Its Use for Functional Assessment of the Adeno-associated Virus Assembly-activating Proteins

Article DOI: 10.3791/56766-v • 14:49 min • June 12th, 2018
June 12th, 2018 •
1, 1, 1, 1,2
1Department of Molecular and Medical Genetics, Oregon Health & Science University, 2Department of Molecular Microbiology and Immunology, Oregon Health & Science University

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This manuscript details a straightforward dot blot assay for quantitation of adeno-associated virus (AAV) titers and its application to study the role of assembly-activating proteins (AAPs), a novel class of non-structural viral proteins found in all AAV serotypes, in promoting the assembly of capsids derived from cognate and heterologous AAV serotypes.

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Keywords: Quantitative Dot Blot AAV Titration AAV Assembly-activating Proteins AAV Vector Quantitation HEK 293 Cell Transfection Virus Production AAV Recovery Cell Debris Pelleting
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