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Journal / Genetics / Semi-quantitative Detection of RNA-dependent RNA…
JoVE Journal
Genetics

This Conteúdo is Open Access.

JoVE Journal Genetics
Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein
 

Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein

Article DOI: 10.3791/57021-v • 08:26 min • June 12th, 2018
June 12th, 2018 •
*1, *1, 1, 1, 1
1Division of Cancer Stem Cell, National Cancer Center Research Institute
* These authors contributed equally

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Human telomerase reverse transcriptase (TERT) synthesizes not only telomeric DNA but also double-stranded RNA through RNA-dependent RNA polymerase activity. Here, we describe a newly established assay to detect RNA-dependent RNA polymerase activity of endogenous TERT.

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Keywords: RNA-dependent RNA Polymerase TERT RNA Biology RdRP Activity HeLa Cells Cell Lysis Immunoprecipitation Protein Purification Micrococcal Nuclease Semi-quantitative Detection
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