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JoVE Journal Biochemistry
Fractionation for Resolution of Soluble and Insoluble Huntingtin Species
 

Fractionation for Resolution of Soluble and Insoluble Huntingtin Species

Article DOI: 10.3791/57082-v • 07:08 min • February 27th, 2018
February 27th, 2018 •
1,2, 2,3, 4, 1,2,3
1Department of Psychiatry and Human Behavior, University of California Irvine, 2UCI MIND, University of California Irvine, 3Department of Neurobiology and Behavior, University of California Irvine, 4Board of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center

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A method is described for fractionation of insoluble and soluble mutant huntingtin species from mouse brain and cell culture. The method described is useful for characterization and quantification of huntingtin protein flux and aids in analyzing protein homeostasis in disease pathogenesis and in the presence of perturbations

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Fractionation Resolution Soluble Insoluble Huntingtin Species Visualization Characterization Quantification Protein Flux Protein Homeostasis Huntington's Disease Therapeutic Interventions Biochemical Changes Misfolding Diseases Parkinson's Disease Alzheimer's Disease Lysis Buffer Inhibitors Tissue Homogenization
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