Journal
/
Bioengenharia
/
Gel-seq: A Method for Simultaneous Sequencing Library Preparation of DNA and RNA Using Hydrogel Matrices
JoVE Journal
Bioengenharia
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Bioengenharia
Gel-seq: A Method for Simultaneous Sequencing Library Preparation of DNA and RNA Using Hydrogel Matrices

Gel-seq: A Method for Simultaneous Sequencing Library Preparation of DNA and RNA Using Hydrogel Matrices

DOI:
10.3791/57315-v

•

09:19 min

•

March 26, 2018

•

, , , ,
1Department of Engineering,University of San Diego, 2Department of Bioengineering,University of California, San Diego, 3Department of Mechanical and Aerospace Engineering,University of California, San Diego

Capítulos

  • 00:04Título
  • 00:42Gel-seq Cassette Fabrication
  • 03:12Gel Separation
  • 04:36Sample Recovery
  • 06:53Results: Gel-seq Compared with Conventional Methods
  • 08:26Conclusion

Summary

Tadução automática

Tadução automática

Gel-seq enables researchers to simultaneously prepare libraries for both DNA- and RNA-seq at negligible added cost starting from 100 - 1000 cells using a simple hydrogel device. This paper presents a detailed approach for the fabrication of the device as well as the biological protocol to generate paired libraries.

Tags

Gel-seqSimultaneous SequencingDNA And RNAHydrogel MatricesNext-generation SequencingGenomic And Transcriptomic InformationFiller GelHigh Density GelLow Density GelGel Electrophoresis CassettePolymer Curing

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/57315?article_type=v

Preparation of Complaint Matrices for Quantifying Cellular Contraction
check_url/pt/57315?article_type=v

An Improved Method for the Preparation of Type I Collagen From Skin
check_url/pt/57315?article_type=v

Synthesis of an Intein-mediated Artificial Protein Hydrogel
check_url/pt/57315?article_type=v

Bioprinting Cellularized Constructs Using a Tissue-specific Hydrogel Bioink
check_url/pt/57315?article_type=v

Fabricating a Kidney Cortex Extracellular Matrix-Derived Hydrogel
check_url/pt/57315?article_type=v

Ultra-long Read Sequencing for Whole Genomic DNA Analysis
check_url/pt/57315?article_type=v

High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing
check_url/pt/57315?article_type=v

Simultaneous Quantification of Selected Kynurenines Analyzed by Liquid Chromatography-Mass Spectrometry in Medium Collected from Cancer Cell Cultures
check_url/pt/57315?article_type=v

Simple, Affordable, and Modular Patterning of Cells using DNA
check_url/pt/57315?article_type=v

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Read Article