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Gel-seq: A Method for Simultaneous Sequencing Library Preparation of DNA and RNA Using Hydrogel Matrices
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Bioengineering
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JoVE Journal Bioengineering
Gel-seq: A Method for Simultaneous Sequencing Library Preparation of DNA and RNA Using Hydrogel Matrices

Gel-seq: A Method for Simultaneous Sequencing Library Preparation of DNA and RNA Using Hydrogel Matrices

DOI:
10.3791/57315-v

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09:19 min

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March 26, 2018

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, , , ,
1Department of Engineering,University of San Diego, 2Department of Bioengineering,University of California, San Diego, 3Department of Mechanical and Aerospace Engineering,University of California, San Diego

Chapters

  • 00:04Title
  • 00:42Gel-seq Cassette Fabrication
  • 03:12Gel Separation
  • 04:36Sample Recovery
  • 06:53Results: Gel-seq Compared with Conventional Methods
  • 08:26Conclusion

Summary

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Gel-seq enables researchers to simultaneously prepare libraries for both DNA- and RNA-seq at negligible added cost starting from 100 - 1000 cells using a simple hydrogel device. This paper presents a detailed approach for the fabrication of the device as well as the biological protocol to generate paired libraries.

Tags

Gel-seqSimultaneous SequencingDNA And RNAHydrogel MatricesNext-generation SequencingGenomic And Transcriptomic InformationFiller GelHigh Density GelLow Density GelGel Electrophoresis CassettePolymer Curing

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