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凝胶序列: 一种同时测序库的方法用水凝胶基质制备 DNA 和 RNA
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Bioengineering
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JoVE Journal Bioengineering
Gel-seq: A Method for Simultaneous Sequencing Library Preparation of DNA and RNA Using Hydrogel Matrices
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凝胶序列: 一种同时测序库的方法用水凝胶基质制备 DNA 和 RNA

DOI:
10.3791/57315-v

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09:19 min

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March 26, 2018

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, , , ,
1Department of Engineering,University of San Diego, 2Department of Bioengineering,University of California, San Diego, 3Department of Mechanical and Aerospace Engineering,University of California, San Diego

Chapters

  • 00:04Title
  • 00:42Gel-seq Cassette Fabrication
  • 03:12Gel Separation
  • 04:36Sample Recovery
  • 06:53Results: Gel-seq Compared with Conventional Methods
  • 08:26Conclusion

Summary

Automatic Translation

Automatic Translation

凝胶序列使研究人员能够同时为 DNA 和 RNA 的存储提供从 100-1000 细胞开始使用简单的水凝胶装置的可忽略的额外成本。本文给出了该装置的制作方法, 以及生成配对库的生物协议。

Tags

Gel-seqSimultaneous SequencingDNA And RNAHydrogel MatricesNext-generation SequencingGenomic And Transcriptomic InformationFiller GelHigh Density GelLow Density GelGel Electrophoresis CassettePolymer Curing

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