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Journal / Neuroscience / Isolation and RNA Extraction of Neurons,…
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Neuroscience

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JoVE Journal Neuroscience
Isolation and RNA Extraction of Neurons, Macrophages and Microglia from Larval Zebrafish Brains
 

Isolation and RNA Extraction of Neurons, Macrophages and Microglia from Larval Zebrafish Brains

Article DOI: 10.3791/57431-v • 09:20 min • April 27th, 2018
April 27th, 2018 •
1, 1, 1
1Centre for Discovery Brain Sciences, University of Edinburgh

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Resumo
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We present a protocol to isolate neurons, macrophages and microglia from larval zebrafish brains under physiological and pathological conditions. Upon isolation, RNA is extracted from these cells to analyze their gene expression profile. This protocol allows for the collection of high-quality RNA for performing downstream analysis like qPCR and transcriptomics.

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Keywords: Isolation RNA Extraction Neurons Macrophages Microglia Larval Zebrafish Brains Transgenic Zebrafish Live Imaging Gene Expression Cell Sorting Central Nervous System PTU Fluorescent Stereomicroscope GFP DsRED Tricaine Homogenizer Media A Tissue Disruption RNA Purification QPCR Transcriptomics
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