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JoVE Journal Neuroscience
Identification of Dopamine D1-Alpha Receptor Within Rodent Nucleus Accumbens by an Innovative RNA In Situ Detection Technology
 

Identification of Dopamine D1-Alpha Receptor Within Rodent Nucleus Accumbens by an Innovative RNA In Situ Detection Technology

Article DOI: 10.3791/57444-v • 07:25 min • March 27th, 2018
March 27th, 2018 •
1, 1, 1, 1, 1
1Program in Behavioral Neuroscience, Department of Psychology, University of South Carolina

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Identification of dopamine D1-alpha receptor in the nucleus accumbens is critical for clarifying D1 receptor dysfunction during a central nervous system disease. We performed a novel RNA in situ hybridization assay to visualize single RNA molecules in a specific brain area.

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Keywords: RNA In-situ Hybridization Dopamine D1-alpha Receptor Nucleus Accumbens Central Nervous System Disease Dopaminergic Dysfunction Target MRNA Brain Sectioning Pre-treatment Multiplex Assay Tissue Preparation
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