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JoVE Journal Biochemistry
Toeprinting Analysis of Translation Initiation Complex Formation on Mammalian mRNAs
 

Toeprinting Analysis of Translation Initiation Complex Formation on Mammalian mRNAs

Article DOI: 10.3791/57519-v • 10:37 min • May 10th, 2018
May 10th, 2018 •
1, 1,2,3
1Department of Chemistry and Biochemistry, Alberta RNA Research and Training Institute, University of Lethbridge, 2Department of Neuroscience and the Canadian Centre for Behavioral Neuroscience (CCBN), University of Lethbridge, 3Arnie Charbonneau Cancer Institute, University of Calgary

Capítulos

Resumo
Automatic Translation

Toeprinting aims to measure the ability of in vitro transcribed RNA to form translation initiation complexes with ribosomes under a variety of conditions. This protocol describes a method for toeprinting mammalian RNA and can be used to study both cap-dependent and IRES-driven translation.

Tags

Keywords: Toeprinting Analysis Translation Initiation Complex Mammalian MRNAs IRES RNA Purification Rabbit Reticulocyte Lysate RNase Inhibitor ATP GMP-PNP DTT Spermidine
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