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JoVE Journal Developmental Biology
Efficient Generation of Pancreas/Duodenum Homeobox Protein 1+ Posterior Foregut/Pancreatic Progenitors from hPSCs in Adhesion Cultures
 

Efficient Generation of Pancreas/Duodenum Homeobox Protein 1+ Posterior Foregut/Pancreatic Progenitors from hPSCs in Adhesion Cultures

Article DOI: 10.3791/57641-v • 08:32 min • March 27th, 2019
March 27th, 2019 •
1, 1, 1, 1
1Center for iPS Cell Research and Application (CiRA), Kyoto University

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Here, we present a detailed protocol to differentiate human pluripotent stem cells (hPSCs) into pancreas/duodenum homeobox protein 1+ (PDX1+) cells for the generation of pancreatic lineages based on the non-colony type monolayer growth of dissociated single cells. This method is suitable for producing homogenous hPSC-derived cells, genetic manipulation and screening.

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Keywords: HPSCs Adhesion Culture Basement Membrane Matrix EDTA Y27632 Cell Dissociation Cell Counting Stage 1A Medium Pancreatic Progenitors
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