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Journal / Neuroscience / 健康脑-垂体切片对硬骨鱼类鱼垂体细胞电生理的研究…
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JoVE Journal Neuroscience
健康脑-垂体切片对硬骨鱼类鱼垂体细胞电生理的研究
 
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健康脑-垂体切片对硬骨鱼类鱼垂体细胞电生理的研究

Article DOI: 10.3791/57790-v • 07:14 min • August 16th, 2018
August 16th, 2018 •
*1, *1, 1
1Department of Basic Sciences and Aquatic Medicine, Faculty of Veterinary Medicine, Norwegian University of Life Sciences
* These authors contributed equally

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本文介绍了利用硬骨鱼类鱼鳉 (Oryzias latipes) 制作可行的脑-垂体组织切片的优化协议, 然后用膜片钳技术进行垂体细胞的电生理记录, 并穿孔补丁配置。

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神经科学 问题 138 脑垂体 组织切片 电生理学 鱼 补丁钳 内分泌细胞
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