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JoVE Journal Developmental Biology
Defined Xeno-free and Feeder-free Culture Conditions for the Generation of Human iPSC-derived Retinal Cell Models
 

Defined Xeno-free and Feeder-free Culture Conditions for the Generation of Human iPSC-derived Retinal Cell Models

Article DOI: 10.3791/57795-v • 11:29 min • September 6th, 2018
September 6th, 2018 •
*1, *1, 1, 1, 1
1Institut de la Vision, Sorbonne Université, INSERM, CNRS, F-75012 Paris, France
* These authors contributed equally

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Resumo
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The production of specialized retinal cells from pluripotent stem cells is a turning point in the development of stem cell-based therapy for retinal diseases. The present paper describes a simple method for an efficient generation of retinal organoids and retinal pigmented epithelium for basic, translational, and clinical research.

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Keywords: IPS Cells Retinal Cell Models Retinal Organoids Retinal Pigmented Epithelial Cells RPE Cells Xeno-free Feeder-free Differentiation Culture Conditions Cell Isolation Floating Culture Pro B-27 Medium Pro N-2 Medium FGF2
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