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Large-scale Three-dimensional Imaging of Cellular Organization in the Mouse Neocortex
 

Large-scale Three-dimensional Imaging of Cellular Organization in the Mouse Neocortex

Article DOI: 10.3791/58027-v • 09:55 min • September 5th, 2018
September 5th, 2018 •
1, 1,2, 1, 1
1RIKEN Brain Science Institute, 2Tokyo Metropolitan Institute of Medical Science

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Here we describe a procedure for tissue clearing, fluorescent labeling, and large-scale imaging of mouse brain tissue which, thereby, enables visualization of the three-dimensional organization of cell types in the neocortex.

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Keywords: 3D Imaging Cellular Organization Mouse Neocortex Neuroanatomy Neural Tracing Cholera Toxin Subunit B Stereotaxic Injection
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