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Whole Cell Electrophysiology of Primary Cultured Murine Enterochromaffin Cells
 

Whole Cell Electrophysiology of Primary Cultured Murine Enterochromaffin Cells

Article DOI: 10.3791/58112-v • 10:04 min • September 26th, 2018
September 26th, 2018 •
*1, *1, 2, 2, 1, 1
1Enteric Neuroscience Program, Division of Gastroenterology & Hepatology,Department of Physiology & Biomedical Engineering, Mayo Clinic, 2Division of Gastroenterology, Department of Medicine, University of Massachusetts Medical School
* These authors contributed equally

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Resumo
Automatic Translation

Enterochromaffin (EC) cells comprise a small subset of gastrointestinal epithelial cells. EC cells are electrically excitable and release serotonin, yet difficulties in culturing and identifying EC cells have limited physiological studies. The method presented here establishes a primary culture model amenable to examination of single EC cells by electrophysiology.

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Keywords: Whole Cell Electrophysiology Primary Cultured Murine Enterochromaffin Cells Enteric Neuroscience Gastrointestinal Physiology Enterochromaffin Cell Excitability Gastrointestinal Hormone Release Single-cell Techniques Electrophysiology Intestinal Tissue PBS Microdissection Tissue Mincing Collagenase Digestion
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