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Bioengineering

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JoVE Journal Bioengineering
Use of High-Throughput Automated Microbioreactor System for Production of Model IgG1 in CHO Cells
 

Use of High-Throughput Automated Microbioreactor System for Production of Model IgG1 in CHO Cells

Article DOI: 10.3791/58231-v • 08:15 min • September 28th, 2018
September 28th, 2018 •
1, 1, 1, 1, 1, 1, 1, 1, 1
1Center for Drug Evaluation and Research, Office of Product Quality, Office of Biotechnology Products, Division of Biotechnology Review and Research II, U.S. Food and Drug Administration

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Resumo
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A detailed protocol for the concurrent operation of 48 parallel cell cultures under varied conditions in a microbioreactor system is presented. Cell culture process, harvest and subsequent antibody titer analysis are described.

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Keywords: High-throughput Automated Microbioreactor System IgG1 Production CHO Cells Cell Line Characterization Process Optimization Shake Flasks Automation Small-scale Studies Cell Culture Subculturing Cell Counter Microbioreactor Software Temperature Control Dissolved Oxygen PH Monitoring
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