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JoVE Journal Cancer Research
Phospho Flow Cytometry with Fluorescent Cell Barcoding for Single Cell Signaling Analysis and Biomarker Discovery
 

Phospho Flow Cytometry with Fluorescent Cell Barcoding for Single Cell Signaling Analysis and Biomarker Discovery

Article DOI: 10.3791/58386-v • 08:38 min • October 4th, 2018
October 4th, 2018 •
1,2
1Centre for Molecular Medicine Norway (NCMM), Nordic EMBL Partnership, University of Oslo, 2K. G. Jebsen Centre for B cell malignancies and K. G. Jebsen Centre for Cancer Immunotherapy, University of Oslo

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Here, a protocol for medium- to high-throughput analysis of protein phosphorylation events at the cellular level is presented. Phospho flow cytometry is a powerful approach to characterize signaling aberrations, identify and validate biomarkers, and assess pharmacodynamics.

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Phospho Flow Cytometry Fluorescent Cell Barcoding Single Cell Signaling Analysis Biomarker Discovery Signaling Pathways Stimuli Drugs Medium To High Throughput Fashion RPMI 16/40 Medium 96 Well V-bottom Plate Fixed Buffer One Control Sample Anti-IgM Simulation Time Course Fix Plate Water Bath PBS Wash Barcoding Reagents
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