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JoVE Journal Cancer Research
A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer
 

A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer

Article DOI: 10.3791/64304-v • 10:46 min • September 13th, 2022
September 13th, 2022 •
1,2, 1,2, 1,2, 1,2, 1,2, 1,2
1School of Medicine, Deakin University, 2Institute for Mental and Physical Health and Clinical Translation, School of Medicine, Deakin University

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A necessary step in anticancer aptamer development is to test its binding to the target. We demonstrate a flow cytometric-based assay to study this binding, emphasizing the importance of including a negative control aptamer and cancer cells that are positive or negative for that particular protein. 

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Keywords: Flow Cytometry Cell Surface Protein Aptamer Anticancer Selectivity Specificity Immunophenotyping Multi-parametric Cell Culture Trypsinization Cell Counting Cell Stabilization Blocking Buffer Aptamer Folding
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