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Journal / Genetics / En tilpasselig protokol for streng forsamling…
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Genetics

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JoVE Journal Genetics
En tilpasselig protokol for streng forsamling gRNA kloning (STAgR)
 
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En tilpasselig protokol for streng forsamling gRNA kloning (STAgR)

Article DOI: 10.3791/58556-v • 10:00 min • December 26th, 2018
December 26th, 2018 •
1,2, 1,2, 3, 3, 2,4, 1,2
1MCN Junior Research Group, Munich Center for Neurosciences, Ludwig Maximilian Universitat, BioMedical Center, 2Institute of Stem Cell Research, Helmholtz Zentrum, German Research Center for Environmental Health, 3Institute of Developmental Genetics, Helmholtz Zentrum, German Research Center for Environmental Health, 4Physiological Genomics, Ludwig Maximilian Universitat, BioMedical Center

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Vi præsenterer her, streng forsamling gRNA kloning (STAgR), en metode til let multiplex gRNA vektorer for CRISPR/Cas9 tilgange. STAgR gør gRNA multiplexing enkle, effektive og kan tilpasses.

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Genetik sag 142 CRISPR Cas9 dCas9 gRNA kloning gRNA multiplexing genom-redigering redigering af transkriptom
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