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Isolation of Primary Murine Skeletal Muscle Microvascular Endothelial Cells
 

Isolation of Primary Murine Skeletal Muscle Microvascular Endothelial Cells

Article DOI: 10.3791/58901-v • 11:57 min • March 6th, 2019
March 6th, 2019 •
*1, *1, 1, *1, *1
1Institute for Translational Neurology and Neurology Clinic, University of Muenster
* These authors contributed equally

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Resumo
Automatic Translation

Microvascular endothelial cells of skeletal muscles (MMEC) shape the inner wall of muscle capillaries and regulate both, exchange of fluids/molecules and migration of (immune) cells between muscle tissue and blood. Isolation of primary murine MMEC, as described here, enables comprehensive in vitro investigations of the "myovascular unit".

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Keywords: Primary Murine Skeletal Muscle Microvascular Endothelial Cells Isolation Tissue Dissociation Cell Culture Digestion Tissue Processing
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