Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Cancer Research / Yeast As a Chassis for Developing Functional…
JoVE Journal
Cancer Research
Author Produced

É necessário ter uma assinatura JoVE para assistir este Faça login ou comece sua avaliação gratuita.

JoVE Journal Cancer Research
Yeast As a Chassis for Developing Functional Assays to Study Human P53
 

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Article DOI: 10.3791/59071-v • 14:57 min • August 4th, 2019
August 4th, 2019 •
1, 2, 3, *3, *1, *2
1Mutagenesis and Cancer Prevention Unit, Ospedale Policlinico San Martino, 2Department CIBIO, University of Trento, 3LAQV/REQUIMTE, Laboratory of Microbiology, Faculty of Pharmacy, University of Porto
* These authors contributed equally

Capítulos

Resumo
Automatic Translation

Presented here are four protocols to construct and exploit yeast Saccharomyces cerevisiae reporter strains to study human P53 transactivation potential, impacts of its various cancer-associated mutations, co-expressed interacting proteins, and the effects of specific small molecules.

Tags

Yeast Chassis Functional Assays Study Human P53 Transcription Transcription Factor Cofactor Cis-acting Elements Response Elements DNA Sequencing Modulation Target Genes Protocol Transactivation Functions Model System Color Reporter Gene Luciferase Cell Growth Quantification Experimental Steps Versatility Construction Of Reporter Yeast Strains Specific P53 Response Element Adenine-2 Gene Firefly Luciferase Gene Transformation Lithium Acetate-based Protocol 5-FOA Plates Incubation At 30 Degrees Replica Plate YPDA Plates G418
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter