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JoVE Journal Biochemistry
Fluorescent Visualization of Mango-tagged RNA in Polyacrylamide Gels via a Poststaining Method
 

Fluorescent Visualization of Mango-tagged RNA in Polyacrylamide Gels via a Poststaining Method

Article DOI: 10.3791/59112-v • 06:06 min • June 21st, 2019
June 21st, 2019 •
*1, *1, 1
1Department of Molecular Biology and Biochemistry, Simon Fraser University
* These authors contributed equally

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Resumo
Automatic Translation

Here we present a sensitive, rapid, and discriminating post-gel staining method to image RNAs tagged with RNA Mango aptamers I, II, III, or IV, using either native or denaturing polyacrylamide gel electrophoresis (PAGE) gels. After running standard PAGE gels, Mango-tagged RNA can be easily stained with TO1-Biotin and then analyzed using commonly available fluorescence readers.

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Keywords: Mango-tagged RNA Polyacrylamide Gels Poststaining Method Fluorescent Visualization RNA Purification RNA Tracking RNA Visualization Denaturing Gel Gel Electrophoresis RNA Samples Gel Staining Solution Gel Imaging
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