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JoVE Journal Genetics
Enhanced Yeast One-hybrid Screens To Identify Transcription Factor Binding To Human DNA Sequences
 

Enhanced Yeast One-hybrid Screens To Identify Transcription Factor Binding To Human DNA Sequences

Article DOI: 10.3791/59192-v • 11:25 min • February 11th, 2019
February 11th, 2019 •
*1, *1, *1, 1,2
1Department of Biology, Boston University, 2Bioinformatics Program, Boston University
* These authors contributed equally

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Resumo
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Here, we present an enhanced yeast one-hybrid screening protocol to identify the transcription factors (TFs) that can bind to a human DNA region of interest. This method uses a high-throughput screening pipeline that can interrogate the binding of >1,000 TFs in a single experiment.

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Keywords: Transcription Factor Yeast One-hybrid DNA Sequence Functional Genomics Gene Promoters Enhancers Non-coding Variants Array Robot High Density 96-well Plates 384-well Plates 1536-well Plates Colony Array Sc Minus Tryptophan
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