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JoVE Journal Cancer Research
Patient-derived Orthotopic Xenograft Models for Human Urothelial Cell Carcinoma and Colorectal Cancer Tumor Growth and Spontaneous Metastasis
 

Patient-derived Orthotopic Xenograft Models for Human Urothelial Cell Carcinoma and Colorectal Cancer Tumor Growth and Spontaneous Metastasis

Article DOI: 10.3791/59223-v • 09:28 min • May 12th, 2019
May 12th, 2019 •
1, 1, 1, 2, 3, 3, 1, 2,4, 2,4, 3,4, 1,4
1Laboratory of Translational Cancer Research, Institute for Translational Research, Ochsner Clinic Foundation, 2Department of Urology, Ochsner Clinic Foundation, 3Department of Colon and Rectal Surgery, Ochsner Clinic Foundation, 4Ochsner Clinical School, University of Queensland, School of Medicine

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This protocol describes the generation of patient-derived orthotopic xenograft models by intra-vesically instilling high-grade urothelial cell carcinoma cells or intra-rectally injecting colorectal cancer cells into non-obese diabetic/severe combined immunodeficiency (NOD/SCID) mice for primary tumor growth and spontaneous metastases under the influence of lymph node stromal cells, which mimics the progression of human metastatic diseases.

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Keywords: Orthotopic Xenograft Urothelial Cell Carcinoma Colorectal Cancer Tumor Growth Spontaneous Metastasis Lymph Node Tumor Implantation Metastatic Patterns Luciferase Collagenase Hyaluronidase Deoxyribonuclease Cell Strainer NOD-SCID Mouse Electrocautery
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