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JoVE Journal Biochemistry
Imaging of Extracellular Vesicles by Atomic Force Microscopy
 

Imaging of Extracellular Vesicles by Atomic Force Microscopy

Article DOI: 10.3791/59254-v • 10:11 min • September 11th, 2019
September 11th, 2019 •
1,2, 3,4
1Department of Chemical Engineering, University of Utah, 2The Nano Institute of Utah, University of Utah, 3Center for Photonics and Quantum Materials, Skolkovo Institute of Science and Technology, 4Biopharmaceutical Cluster 'Northern', Moscow Institute of Physics and Technology

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Resumo
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A step-by-step procedure is described for label-free immobilization of exosomes and extracellular vesicles from liquid samples and their imaging by atomic force microscopy (AFM). The AFM images are used to estimate the size of the vesicles in the solution and characterize other biophysical properties. 

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AFM Imaging Extracellular Vesicles Hydrated And Desiccated Forms Electrostatic Immobilization Surface Scanning Vesical Identification Data Analysis Interpretation Shape Distortion Vesicle Sizing CryoTEM Imaging Dry Samples Characterization Bio-fluid Isolation Mica Disc Attachment Cleaving Mica Disc Surface Modification With Nickel II Chloride Solution Deionized Water Washing Dry Nitrogen Drying AFM Specimen Placement
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