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Visualization of Endogenous Mitophagy Complexes In Situ in Human Pancreatic Beta Cells Utilizing Proximity Ligation Assay
 

Visualization of Endogenous Mitophagy Complexes In Situ in Human Pancreatic Beta Cells Utilizing Proximity Ligation Assay

Article DOI: 10.3791/59398-v • 08:40 min • May 2nd, 2019
May 2nd, 2019 •
1, 1,2
1Department of Internal Medicine, Division of Metabolism, Endocrinology and Diabetes, University of Michigan, Ann Arbor, 2VA Ann Arbor Healthcare System

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Resumo
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This protocol outlines a method for quantitative analysis of mitophagy protein complex formation specifically in beta cells from primary human islet samples. This technique thus allows analysis of mitophagy from limited biological material, which are crucial in precious human pancreatic beta cell samples.

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Keywords: Mitophagy Proximity Ligation Assay Human Pancreatic Beta Cells Endogenous Mitophagy Complexes Immunohistochemistry Ubiquitin-specific Peptidase 8 Neuregulin Receptor Degradation Protein-1 Beta Cell Marker
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