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JoVE Journal Cancer Research
Establishment and Characterization of Three Afatinib-resistant Lung Adenocarcinoma PC-9 Cell Lines Developed with Increasing Doses of Afatinib
 

Establishment and Characterization of Three Afatinib-resistant Lung Adenocarcinoma PC-9 Cell Lines Developed with Increasing Doses of Afatinib

Article DOI: 10.3791/59473-v • 09:38 min • June 26th, 2019
June 26th, 2019 •
1, 1, 2, 1, 3
1Advanced Cancer Translational Research Institute, Showa University, 2Division of Breast Surgical Oncology, Department of Surgery, Showa University School of Medicine, 3Division of Allergology and Respiratory Medicine, Department of Medicine, Showa University School of Medicine

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Resumo
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A method for establishing afatinib-resistance cell lines from lung adenocarcinoma PC-9 cells was developed, and resistant cells were characterized. The resistant cells can be used to investigate epidermal growth factor receptor tyrosine kinase inhibitor-resistance mechanisms, applicable for patients with non-small cell lung cancer.

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Keywords: Afatinib Resistance Lung Adenocarcinoma PC-9 Cell Lines Acquired Resistance Stepwise Dose Escalation MTT Assay Cell Culture Subconfluency Dose-dependent Resistance
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