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伦蒂病毒介导基因在低附着板中制备的人类伪性沉默

JoVE Journal
Genética

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JoVE Journal Genética

Lentiviral Mediated Gene Silencing in Human Pseudoislet Prepared in Low Attachment Plates

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伦蒂病毒介导基因在低附着板中制备的人类伪性沉默

DOI:

10.3791/59578-v

•

11:24 min

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May 14, 2019

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Siming Liu², Mikako Harata², Joseph A. Promes², Anthony J. Burand³, James A. Ankrum³, Yumi Imai²

¹Department of Internal Medicine, Carver College of Medicine,University of Iowa, ²Fraternal Order of Eagles Diabetes Research Center,University of Iowa, ³Roy J. Carver Department of Biomedical Engineering,University of Iowa

Capítulos

00:04Título
00:42Overnight Culture of Human Islets for Recovery After Shipment
01:32Preparation of Single Cell Suspension from Human Islets
04:03Pseudoislet Formation and Transduction by Lentivirus
08:44Results: Morphological and Functional Studies Using Gene Modified Human Pseudoislets
09:53Conclusion

Summary

Tadução automática

提出了一种从分散的人类胰岛细胞中产生基因修饰的人类伪胰岛的函数,这种细胞由携带短发核糖核酸(shRNA)的慢病毒转导。该协议利用现成的酶和培养容器,易于执行,并产生适合功能和形态学研究的转基因人类假岛。

Tags

Keywords: Lentiviral Gene Silencing Human Pseudoislet Low Attachment Plates Islet Function Gene Expression Modulation SHR8 Transduction Proteolytic Enzyme Collagenolytic Enzyme Cell Dissociation CMRL Medium Fetal Bovine Serum

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