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Journal / Immunology and Infection / Pan-lyssavirus Real Time RT-PCR for Rabies…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Pan-lyssavirus Real Time RT-PCR for Rabies Diagnosis
 

Pan-lyssavirus Real Time RT-PCR for Rabies Diagnosis

Article DOI: 10.3791/59709-v • 06:25 min • July 10th, 2019
July 10th, 2019 •
1, 1, 1, 1, 1,2, 1, 1,2
1Wildlife Zoonoses & Vector-Borne Diseases Research Group, Animal and Plant Health Agency, 2Institute of Infection and Global Health, University of Liverpool

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This real-time RT-PCR using dsDNA intercalating dye is suitable to diagnose lyssavirus infections. The method begins with RNA extracted from rabies suspected ante-mortem or post-mortem samples, detailing master mix preparation, RNA addition, setup of the real-time machine and correct interpretation of results.

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Keywords: Pan-Lyssavirus Real Time RT-PCR Rabies Diagnosis Lyssavirus Genus Molecular Assays RNA Quantification PCR Master Mix Beta-Actin SYBR Green Dissociation Curve
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